In case of nerve transection we observed biochemical and morphological changes in axons. The aim of present study was to examine neurotrophic activity of two important metalloproteinases: MMP-2 and MMP-9. To examine their activity in nerve supernatants, gelatin zymography was used. We concluded that the levels of MMP-2 and MMP-9 expression are increased in 4th, 5th and 6th day after nerve transection. The identification of the molecular mechanism underlying this activity could be the main key for the elaborating of further strategies for repair of the damaged nervous system.