Abstract
Herpes simplex virus type 1 (HSV-1) ICP27 protein is an essential regulator of viral gene expression with roles at various levels of RNA metabolism in the nucleus. Using the tethered function assay, we showed a cytoplasmic activity for ICP27 in directly enhancing mRNA translation in vivo in the absence of other viral factors. The region of ICP27 required for translational stimulation maps to the C terminus. Furthermore, in infected cells, ICP27 is associated with polyribosomes, indicating a function in translation during the lytic cycle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cricetinae
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Female
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Herpesvirus 1, Human / genetics*
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Herpesvirus 1, Human / metabolism*
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Immediate-Early Proteins / chemistry
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Immediate-Early Proteins / genetics*
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Immediate-Early Proteins / metabolism*
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In Vitro Techniques
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Oocytes / metabolism
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Polyribosomes / virology
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Protein Biosynthesis*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Viral / genetics
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RNA, Viral / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Xenopus
Substances
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ICP27 protein, human herpesvirus 1
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Immediate-Early Proteins
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RNA, Messenger
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RNA, Viral
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Recombinant Fusion Proteins