Toxicological evaluation of Media azedarach Linn. was carried out in lower laboratory animals, i.e. rats and mice by oral and intravenous routes. Aqueous and alcoholic extracts were found to be non-toxic uptil a dose of 1500 mg/kg orally in mice and rats. When injected aqueous extract intravenously, LD50 was 395, 580 mg/kg (flowers) and 700, 925 mg/kg (berries) respectively in mice and rats. It was also observed that alcoholic and aqueous extracts of Melia azedarach have a mild CNS sedative effect.