Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets

Vet Microbiol. 2006 May 31;114(3-4):337-44. doi: 10.1016/j.vetmic.2005.12.005. Epub 2006 Jan 18.

Abstract

We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n=7) and clinical (n=3) infected cattle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / chemistry
  • Antigens, Bacterial / immunology*
  • Base Sequence
  • Cattle
  • Cattle Diseases / immunology*
  • Cattle Diseases / microbiology
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Molecular Sequence Data
  • Molecular Weight
  • Mycobacterium avium subsp. paratuberculosis / immunology*
  • Mycobacterium avium subsp. paratuberculosis / metabolism
  • Paratuberculosis / immunology*
  • Paratuberculosis / microbiology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial