The first radiation hybrid map of a perch-like fish: the gilthead seabream (Sparus aurata L)

Genomics. 2006 Jun;87(6):793-800. doi: 10.1016/j.ygeno.2005.11.019. Epub 2006 Jan 18.

Abstract

Among Teleosts, Perciformes are the largest order of fishes and include numerous species of commercial importance. Perciformes also comprise species of primary interest for evolutionary studies and analysis of the sex determination systems and sex chromosome plasticity. Unfortunately, genomics tools and resources for Perciformes remain to be developed. Here, we report the production of a seabream whole-genome radiation hybrid (RH) panel in which quality was ascertained by the construction of a 2-Mb-resolution RH map. The map encompasses 440 markers (288 microsatellites, 82 gene-based markers, and 70 STS) suitable for linkage analysis and comparative mapping studies. Achievement of a RH panel and a whole-genome RH map should contribute to establishing seabream as a fish model among the Perciformes and should be of importance in aquaculture for marker-assisted selection, improvement of growth performance, and disease management. Development of RH maps in a cost-effective manner for other fishes with the described methodology will offer a powerful approach in aquaculture and will provide extended capabilities for comparing vertebrate genome evolution.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Evolution
  • Genetic Markers
  • In Vitro Techniques
  • Models, Genetic
  • Perciformes / classification
  • Perciformes / genetics
  • Radiation Hybrid Mapping / methods*
  • Sea Bream / genetics*
  • Sequence Tagged Sites
  • Species Specificity

Substances

  • Genetic Markers