Abstract
Using MIN6 beta-cells and chromatin immunoprecipitation (ChIP) assays, the chronological sequence of binding of MafA, E47/beta2 and PDX-1 to the insulin promoter in living beta-cells were investigated. All four factors were shown to bind to the mouse insulin 2 promoter in a cyclical manner with a periodicity of approximately 10-15 min. The cyclical binding of MafA, E47 and beta2 was largely unaffected by the glucose or insulin concentration in the media. However, the binding and cycling of PDX-1 was markedly abolished in low glucose (1 mM), and this was reversed in the presence of low concentrations of insulin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Glucose / metabolism
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Homeodomain Proteins / metabolism*
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Humans
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Insulin / genetics*
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Insulin / metabolism
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Insulin-Secreting Cells / cytology
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Insulin-Secreting Cells / metabolism
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Maf Transcription Factors, Large / metabolism*
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic*
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Protein Binding
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Sequence Alignment
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TCF Transcription Factors / metabolism*
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Trans-Activators / metabolism*
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Transcription Factor 7-Like 1 Protein
Substances
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Homeodomain Proteins
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Insulin
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Maf Transcription Factors, Large
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Mafa protein, mouse
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TCF Transcription Factors
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TCF7L1 protein, human
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Tcf7l1 protein, mouse
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Trans-Activators
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Transcription Factor 7-Like 1 Protein
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pancreatic and duodenal homeobox 1 protein
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Glucose