Production of saccharogenic and dextrinogenic amylases by Rhizomucor pusillus A 13.36

J Microbiol. 2005 Dec;43(6):561-8.

Abstract

A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75 degrees C. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60 degrees C, respectively. The saccharogenic activity was inhibited by Ca(2+) while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe(2+) and Cu(2+) Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amylases / biosynthesis*
  • Calcium / pharmacology
  • Copper / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Enzyme Stability
  • Glucose / metabolism
  • Hydrogen-Ion Concentration
  • Iron / pharmacology
  • Maltose / metabolism
  • Manihot / metabolism
  • Oligosaccharides / metabolism
  • Rhizomucor / enzymology*
  • Starch / metabolism
  • Sucrose / metabolism*
  • Temperature
  • Time Factors
  • Trisaccharides / metabolism

Substances

  • Enzyme Inhibitors
  • Oligosaccharides
  • Trisaccharides
  • Sucrose
  • maltotriose
  • Maltose
  • Copper
  • Starch
  • Iron
  • Amylases
  • Glucose
  • Calcium