Molecular analysis of 16S-23S spacer regions of Acetobacter species

Folia Microbiol (Praha). 2005;50(4):288-92. doi: 10.1007/BF02931408.

Abstract

16S-23S rDNA internal transcribed spacer regions (ITS) similarities were determined in 8 Acetobacter and 1 Gluconacetobacter strains. ITS-PCR amplification of the 16S-23S spacers showed 2 products of similar size in 7 strains; only 1 product of similar size was found in the 2 remaining strains. Analysis of the PCR products using restriction endonucleases HaeIII, HpaII and AluI revealed 3 different restriction groups of A. pasteurianus for AluI and HaeIII, and 4 restriction groups for HpaII. ITS nucleotide sequences of all studied strains exhibited a 52-98% similarity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetobacter / classification*
  • Acetobacter / genetics
  • Base Sequence
  • DNA, Bacterial / analysis
  • DNA, Ribosomal Spacer / analysis*
  • Gluconacetobacter / classification*
  • Gluconacetobacter / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA, Ribosomal, 16S / genetics*
  • RNA, Ribosomal, 23S / genetics*
  • Restriction Mapping
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S

Associated data

  • GENBANK/AJ621845
  • GENBANK/AJ626714
  • GENBANK/AJ626715
  • GENBANK/AJ626716
  • GENBANK/AJ626717
  • GENBANK/AJ626718
  • GENBANK/AJ626719
  • GENBANK/AJ626720
  • GENBANK/AJ626721