Abstract
16S-23S rDNA internal transcribed spacer regions (ITS) similarities were determined in 8 Acetobacter and 1 Gluconacetobacter strains. ITS-PCR amplification of the 16S-23S spacers showed 2 products of similar size in 7 strains; only 1 product of similar size was found in the 2 remaining strains. Analysis of the PCR products using restriction endonucleases HaeIII, HpaII and AluI revealed 3 different restriction groups of A. pasteurianus for AluI and HaeIII, and 4 restriction groups for HpaII. ITS nucleotide sequences of all studied strains exhibited a 52-98% similarity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetobacter / classification*
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Acetobacter / genetics
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Base Sequence
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DNA, Bacterial / analysis
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DNA, Ribosomal Spacer / analysis*
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Gluconacetobacter / classification*
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Gluconacetobacter / genetics
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Molecular Sequence Data
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Polymerase Chain Reaction / methods
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RNA, Ribosomal, 16S / genetics*
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RNA, Ribosomal, 23S / genetics*
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Restriction Mapping
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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DNA, Ribosomal Spacer
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RNA, Ribosomal, 16S
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RNA, Ribosomal, 23S
Associated data
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GENBANK/AJ621845
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GENBANK/AJ626714
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GENBANK/AJ626715
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GENBANK/AJ626716
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GENBANK/AJ626717
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GENBANK/AJ626718
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GENBANK/AJ626719
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GENBANK/AJ626720
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GENBANK/AJ626721