Abstract
Numerous sheep with the prion gene (PRNP) that encodes for 171QQ develop scrapie, a neurodegenerative prion disease of sheep. Relatively few cases of scrapie-affected sheep with PRNP genetics that encode for 171RR, however, have been found. Using flow cytometric analysis, statistically fewer PrPc-positive microglia and monocytes were observed from sheep with 171RR PRNP genetics than from sheep with 171QQ PRNP genetics (P<0.05). One possibility for the lack of PrP(Sc) accumulation in brains and lymph nodes of scrapie-exposed sheep with 171RR PRNP genetics is because of fewer PrPc-positive myeloid-derived cells available for conversion of PrPc to PrP(Sc).
MeSH terms
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Animals
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Animals, Newborn
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Cells, Cultured
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Female
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Flow Cytometry / methods
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Genotype
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Glial Fibrillary Acidic Protein / metabolism
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Immunohistochemistry / methods
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Microglia / pathology
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Microglia / ultrastructure
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Monocytes / metabolism
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Myeloid Cells / metabolism*
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Myeloid Cells / ultrastructure
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Plant Lectins / metabolism
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PrPC Proteins / metabolism*
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PrPC Proteins / pathogenicity
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Prions / genetics*
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RNA, Messenger / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Scrapie / genetics
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Scrapie / metabolism*
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Scrapie / pathology*
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Sheep
Substances
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Glial Fibrillary Acidic Protein
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Plant Lectins
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PrPC Proteins
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Prions
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RNA, Messenger
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Ricinus communis agglutinin-1