Succinate semialdehyde dehydrogenase deficiency does not down-regulate gamma-hydroxybutyric acid binding sites in the mouse brain

Ashok K Mehta; Georgianna G Gould; Maneesh Gupta; Lawrence P Carter; K Michael Gibson; Maharaj K Ticku

doi:10.1016/j.ymgme.2005.11.012

Succinate semialdehyde dehydrogenase deficiency does not down-regulate gamma-hydroxybutyric acid binding sites in the mouse brain

Mol Genet Metab. 2006 May;88(1):86-9. doi: 10.1016/j.ymgme.2005.11.012. Epub 2006 Jan 10.

Authors

Ashok K Mehta¹, Georgianna G Gould, Maneesh Gupta, Lawrence P Carter, K Michael Gibson, Maharaj K Ticku

Affiliation

¹ Department of Pharmacology, MC 7764, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.

PMID: 16406647
DOI: 10.1016/j.ymgme.2005.11.012

Abstract

We investigated whether succinate semialdehyde dehydrogenase deficiency alters gamma-hydroxybutyric acid (GHB) receptor characteristics due to elevation of GHB levels in the mouse brain. The membrane homogenate binding and quantitative autoradiography using [3H]NCS-382 revealed no significant changes in the affinity (Kd), receptor density (Bmax), or displacement potency (IC50) in various brain regions of Aldh5a1-/- vs. Aldh5a1+/+ mice.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Benzocycloheptenes / metabolism
Brain / physiology*
Cerebral Cortex / metabolism
Down-Regulation
Hippocampus / metabolism
Mice
Receptors, Cell Surface / metabolism*
Succinate-Semialdehyde Dehydrogenase / deficiency*

Substances

4-hydroxybutyric acid receptor
Benzocycloheptenes
Receptors, Cell Surface
NCS 382
Succinate-Semialdehyde Dehydrogenase

Abstract

Publication types

MeSH terms

Substances

Grants and funding