[Preparation of monoclonal antibodies to the Fc-fragment of human IgG and the use of their based immunoenzyme conjugates]

I V Nikolaenko; A Iu Galkin; G E Raevskaia; T V Kas'ianenko; M I Nereshchenko; E S Donskaia; N Ia Spivak

[Preparation of monoclonal antibodies to the Fc-fragment of human IgG and the use of their based immunoenzyme conjugates]

Klin Lab Diagn. 2005 Nov:(11):8-11.

[Article in Russian]

Authors

I V Nikolaenko, A Iu Galkin, G E Raevskaia, T V Kas'ianenko, M I Nereshchenko, E S Donskaia, N Ia Spivak

PMID: 16404968

Abstract

An original kit containing 17 clones of hybridomas, the producers of monoclonal antibodies (MCAb) against the Fc-fragments of human IgG, has been obtained. The possibility of using horseradish peroxidase conjugates of obtained MCAb as a part of ELISA test systems (Diaproph-Med, Ukraine) was comparatively studied. The application of peroxidase conjugates of two monoclonal antibodies (156C10 and 153H11) as part of five diagnostic kits was shown to provide a high specificity and a high sensitivity.

Publication types

Evaluation Study

MeSH terms

Antibodies, Monoclonal / biosynthesis
Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / immunology*
Enzyme-Linked Immunosorbent Assay*
Horseradish Peroxidase / chemistry
Humans
Hybridomas / metabolism
Immunoglobulin Fc Fragments / blood*
Immunoglobulin G / blood*
Reagent Kits, Diagnostic*
Sensitivity and Specificity

Substances

Antibodies, Monoclonal
Immunoglobulin Fc Fragments
Immunoglobulin G
Reagent Kits, Diagnostic
Horseradish Peroxidase