Epoxide hydrolases: structure, function, mechanism, and assay

Methods Enzymol. 2005:400:569-88. doi: 10.1016/S0076-6879(05)00032-7.

Abstract

Epoxide hydrolases are a class of enzymes important in the detoxification of genotoxic compounds, as well as in the control of physiological signaling molecules. This chapter gives an overview on the function, structure, and enzymatic mechanism of structurally characterized epoxide hydrolases and describes selected assays for the quantification of epoxide hydrolase activity.

Publication types

  • Review

MeSH terms

  • Animals
  • Biological Assay / methods*
  • Epoxide Hydrolases / chemistry*
  • Epoxide Hydrolases / genetics
  • Epoxide Hydrolases / metabolism*
  • Humans
  • Inactivation, Metabolic
  • Molecular Structure
  • Protein Folding
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Stearic Acids / analysis
  • Stearic Acids / metabolism
  • Xenobiotics / metabolism

Substances

  • Recombinant Proteins
  • Stearic Acids
  • Xenobiotics
  • 9,10-epoxystearic acid
  • Epoxide Hydrolases
  • leukotriene A4 hydrolase