Abstract
We describe the covalent post-modification of a hydrogen-bonded assembly with the subsequent formation of a potent transmembrane Na+ ion transporter. Olefin metathesis is used to cross-link all 16 guanosine subunits in a lipophilic G-quadruplex. The resulting unimolecular G-quadruplex folds in the environment of a phospholipid membrane and functions as a Na+ ion transporter as judged by fluorescence and 23Na NMR transport assays.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Cations, Monovalent
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Circular Dichroism
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DNA / chemistry*
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DNA / metabolism
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G-Quadruplexes
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Guanine / analogs & derivatives*
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Guanine / metabolism
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Ion Transport
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Lipid Bilayers / chemistry
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Lipid Bilayers / metabolism
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Membranes, Artificial
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Nuclear Magnetic Resonance, Biomolecular
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Sodium / chemistry*
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Sodium / metabolism
Substances
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Cations, Monovalent
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Lipid Bilayers
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Membranes, Artificial
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Guanine
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DNA
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Sodium