Termination-free prokaryotic protein translation by using anticodon-adjusted E. coli tRNASer as unified suppressors of the UAA/UGA/UAG stop codons. Read-through ribosome display of full-length DHFR with translated UTR as a buried spacer arm

Chembiochem. 2006 Feb;7(2):249-52. doi: 10.1002/cbic.200500397.

Authors

Atsushi Ogawa¹, Shinsuke Sando, Yasuhiro Aoyama

Affiliation

¹ Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.

PMID: 16381047
DOI: 10.1002/cbic.200500397

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anticodon*
Codon, Terminator*
Escherichia coli / enzymology
Escherichia coli / genetics
Macromolecular Substances
Nucleic Acid Conformation
Open Reading Frames
Peptide Chain Termination, Translational*
Protein Biosynthesis*
RNA, Transfer, Ser / metabolism*
Ribosomes / metabolism
Tetrahydrofolate Dehydrogenase / genetics*
Tetrahydrofolate Dehydrogenase / metabolism
Untranslated Regions*

Substances

Anticodon
Codon, Terminator
Macromolecular Substances
RNA, Transfer, Ser
Untranslated Regions
Tetrahydrofolate Dehydrogenase