[Research on the polymorphism of heart fatty acid-binding protein gene in Shanxi pig breeds and their crossbred populations using PCR-RFLP]

Yi Chuan. 2005 Nov;27(6):882-6.
[Article in Chinese]

Abstract

The genetic variation in 5' - upstream (Hinf I -RFLP)and the second intron (Hinf I *-RFLP, Hae III-RFLP)of heart fatty acid-binding protein(H-FABP)gene were detected with PCR-RFLP in 286 pigs including Mashen, Shanxi white pig and their crossbred populations. The results showed as follows: (1)Mashen presented only DD genotype while other populations varied,and Mashen crossbred populations had only 2 genotypes(DD, Dd) at the Hae III-RFLP site; (2)At the Hinf I -RFLP site of the 5' -upstream region, the crossbred population of Shanxi white pig and Duroc presented only HH genotype while other populations varied. Frequency of h allele in Mashen was 0.9727. (3)At the Hinf I *-RFLP site of the second intron, only Mashen presented 2 genotypes (BB, Bb), and frequency of B allele was 0.9667. (4)At the Hae III-RFLP and Hinf I *-RFLP sites, all populations were in Hardy-weinberg equilibrium.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Crosses, Genetic
  • Fatty Acid-Binding Proteins / genetics*
  • Female
  • Gene Frequency
  • Genotype
  • Linkage Disequilibrium
  • Male
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length
  • Swine / classification
  • Swine / genetics*

Substances

  • Fatty Acid-Binding Proteins