Abstract
The molecular events that regulate phagocytosis, an important innate immune response, in invertebrate defence cells (haemocytes) are poorly understood. Lymnaea stagnalis haemocytes were used as a model to elucidate the role of cell signalling pathways in phagocytosis by molluscan defence cells. The phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002, significantly impaired haemocyte phagocytic activity in a dose-responsive manner with 10 microM LY294002 reducing internalization of fluorescent-conjugated Escherichia coli by 62% (P < or = 0.001). In contrast, the protein kinase A (PKA) inhibitor KT5720 was without effect. Therefore, PI3-K, but not PKA, appears to control phagocytosis by haemocytes in these gastropod molluscs.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Carbazoles / pharmacology
-
Chromones / pharmacology
-
Cyclic AMP-Dependent Protein Kinases / drug effects
-
Cyclic AMP-Dependent Protein Kinases / metabolism*
-
Enzyme Inhibitors / pharmacology
-
Hemocytes / drug effects
-
Hemocytes / enzymology*
-
Indoles / pharmacology
-
Lymnaea / physiology*
-
Morpholines / pharmacology
-
Phagocytosis / drug effects
-
Phagocytosis / physiology*
-
Phosphatidylinositol 3-Kinases / drug effects
-
Phosphatidylinositol 3-Kinases / metabolism*
-
Pyrroles / pharmacology
Substances
-
Carbazoles
-
Chromones
-
Enzyme Inhibitors
-
Indoles
-
Morpholines
-
Pyrroles
-
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
-
KT 5720
-
Phosphatidylinositol 3-Kinases
-
Cyclic AMP-Dependent Protein Kinases