New cell lines from larval fat bodies of Spodoptera exigua: characterization and susceptibility to baculoviruses (Lepidoptera: Noctuidae)

Huan Zhang; Yong-an Zhang; Qilian Qin; Xuan Li; Lin Miao; Yuzhu Wang; Zhongqi Yang; Cui Ding

doi:10.1016/j.jip.2005.10.004

New cell lines from larval fat bodies of Spodoptera exigua: characterization and susceptibility to baculoviruses (Lepidoptera: Noctuidae)

J Invertebr Pathol. 2006 Jan;91(1):9-12. doi: 10.1016/j.jip.2005.10.004. Epub 2005 Dec 22.

Authors

Huan Zhang¹, Yong-an Zhang, Qilian Qin, Xuan Li, Lin Miao, Yuzhu Wang, Zhongqi Yang, Cui Ding

Affiliation

¹ Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China. zhangyab@forestry.ac.cn

PMID: 16376374
DOI: 10.1016/j.jip.2005.10.004

Abstract

Two new cell lines, designated IOZCAS-Spex-II and IOZCAS-Spex-III, were initiated from the fat bodies of larvae of the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. The spherical cells were predominant among the various cell types and measures approximately 15 microm in diameter. The cell lines were mainly composed of tetraploid cells with chromosome numbers ranging from 116 to 131 (n=31). The cell lines were confirmed to have originated from the S. exigua by DAF-PCR technique. They were susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses from S. exigua.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods
Cell Line*
Fat Body / cytology*
Fibroblasts / cytology*
Fibroblasts / virology
Hemocytes / cytology*
Hemocytes / virology
Larva / cytology
Nucleopolyhedroviruses / physiology
Polymerase Chain Reaction
Spodoptera / cytology*
Spodoptera / physiology