Abstract
Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced alpha-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Differentiation / physiology
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Cell Line, Tumor*
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Escherichia coli / pathogenicity*
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / pathology*
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Humans
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Immunohistochemistry
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Organoids / microbiology
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Organoids / pathology*
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Organoids / ultrastructure
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Urothelium / microbiology*
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Urothelium / pathology*
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Urothelium / ultrastructure
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Weightlessness Simulation