Abstract
A wbpP gene encoding a putative UDP-N-acetyl-D-glucosamine C(4) epimerase was identified and cloned from Vibrio vulnificus. The functions of the wbpP gene, assessed by the construction of an isogenic mutant and by evaluating its phenotype changes, demonstrated that WbpP is essential in both the pathogenesis and the capsular polysaccharide biosynthesis of V. vulnificus.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Bacterial Capsules / biosynthesis
-
Bacterial Capsules / genetics
-
Carbohydrate Epimerases / genetics*
-
Carbohydrate Epimerases / physiology
-
Cloning, Molecular
-
Epithelial Cells / microbiology
-
Epithelial Cells / pathology
-
Mice
-
Mice, Inbred ICR
-
Molecular Sequence Data
-
Sequence Analysis, DNA
-
Vibrio Infections / enzymology
-
Vibrio Infections / pathology
-
Vibrio vulnificus / enzymology
-
Vibrio vulnificus / genetics*
-
Vibrio vulnificus / pathogenicity
-
Virulence / genetics
Substances
-
Carbohydrate Epimerases
-
UDP-N-acetylglucosamine 4-epimerase