A derivative of Mycobacterium smegmatis mc(2)155 that lacks the duplicated chromosomal region

Digby F Warner; Gilles Etienne; Xiao-Ming Wang; Limenako G Matsoso; Stephanie S Dawes; Karine Soetaert; Neil G Stoker; Jean Content; Valerie Mizrahi

doi:10.1016/j.tube.2005.10.001

A derivative of Mycobacterium smegmatis mc(2)155 that lacks the duplicated chromosomal region

Tuberculosis (Edinb). 2006 Nov;86(6):438-44. doi: 10.1016/j.tube.2005.10.001. Epub 2005 Dec 15.

Authors

Digby F Warner¹, Gilles Etienne, Xiao-Ming Wang, Limenako G Matsoso, Stephanie S Dawes, Karine Soetaert, Neil G Stoker, Jean Content, Valerie Mizrahi

Affiliation

¹ MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST-NRF, School of Pathology of the University of the Witwatersrand, Johannesburg, South Africa. digby.warner@nhls.ac.za <digby.warner@nhls.ac.za>

PMID: 16359921
DOI: 10.1016/j.tube.2005.10.001

Abstract

The genome of Mycobacterium smegmatis mc(2)155 contains a 56kb duplicated region. We isolated a mutant of mc(2)155 lacking this duplication (DeltaDRKIN). This mutation did not affect the growth rate, surface properties or transformation efficiency of the organism, confirming the potential utility of DeltaDRKIN for the study of genes contained within the duplicated region.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Duplication*
Genes, Bacterial*
Mycobacterium smegmatis / genetics*
Mycobacterium smegmatis / growth & development
Open Reading Frames
Plasmids
Polymerase Chain Reaction / methods
Surface Properties
Transformation, Genetic

Grants and funding

Wellcome Trust/United Kingdom