Abstract
To study the Populus response to an osmotic stress, we have isolated one cDNA encoding a histidine-aspartate kinase (HK1) and four cDNAs encoding histidine-containing phosphotransfer proteins (HPts), HPt1-4. The predicted HK1 protein shares a typical structure with ATHK1 and SLN1 osmosensors. The 4 HPTs are characterized by the histidine phosphotransfer domain. We have shown that HK1 is upregulated during an osmotic stress in hydroponic culture. We have detected an interaction between HK1 and HPt2, using the yeast two-hybrid system. These results suggest the existence of a multi-step phosphorelay pathway probably involved in osmotic stress sensing in Populus.
MeSH terms
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Amino Acid Sequence
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Arabidopsis Proteins / genetics
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Cation Transport Proteins / genetics
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Histidine Kinase
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Intracellular Signaling Peptides and Proteins
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Ion Transport / genetics
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Molecular Sequence Data
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Osmotic Pressure
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Plant Roots / enzymology
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Plant Roots / genetics*
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Populus / enzymology
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Populus / genetics*
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Protein Binding
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Protein Kinases / genetics*
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Protein Kinases / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Sequence Homology, Amino Acid
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Symporters / genetics
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Two-Hybrid System Techniques
Substances
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Arabidopsis Proteins
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Cation Transport Proteins
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HKT1 protein, Arabidopsis
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Intracellular Signaling Peptides and Proteins
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Plant Proteins
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Saccharomyces cerevisiae Proteins
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Symporters
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Protein Kinases
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Histidine Kinase
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SLN1 protein, S cerevisiae