Neural crest cells and patterning of the mammalian dentition

Abstract

The mammalian dentition is composed of serial groups of teeth, each with a distinctive crown and root morphology, highly adapted to its particular masticatory function. In the embryo, generation of individual teeth within the jaws relies upon interactions between ectoderm of the first branchial arch and the neural crest-derived ectomesenchymal cells that migrate into this region from their site of origin along the neural axis. Classic tissue recombination experiments have provided evidence of an essential role of the ectoderm in initiating tooth development; however, the underlying ectomesenchyme rapidly acquires dominance in establishing shape. A key question is how these cells acquire this positional information. One theory suggests that ectomesenchymal cells are pre-patterned with respect to shape generation. Alternatively, this cell population acquires positional information within the first branchial arch itself, following migration. Recent molecular evidence suggests a high degree of plasticity within these ectomesenchymal cells. In particular, signalling molecules within the ectoderm exert a time-dependent influence upon the ectomesenchyme by establishing specific domains of homeobox gene expression. Initially, these ectomesenchymal cells are plastic and able to respond to signalling from the ectoderm, however, this plasticity is rapidly lost and pattern information becomes fixed. Therefore, in the first branchial arch, local regulation between the ectoderm and neural crest-derived ectomesenchyme is crucial in establishing the appropriate tooth shape in the correct region of the jaw.