Abstract
Forty-five GM1-binding peptides were identified using phage-displayed peptides libraries of random peptides. Most have a motif containing a hydrophobic amino acid followed by a serine (S). Based on a GM1-binding assays, two of these GM1-binding peptides (named 15 and 40) were chosen to investigate its immunostimulatory properties when chemically coupled to antigens. Mice intra-nasally (i.n.) vaccinated with some of these complexes developed a better local and systemic antibody response than mice i.n. vaccinated with the respective uncoupled antigens. The efficiency of the complex GM1-binding peptide-antigen strongly depends on the composition and structure of both of the components of the complex.
MeSH terms
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Adjuvants, Immunologic* / administration & dosage
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Administration, Intranasal
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Animals
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Antibodies, Bacterial / blood
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Antibodies, Viral / blood
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Antigens, Bacterial / administration & dosage
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / immunology*
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Antigens, Viral / administration & dosage
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Antigens, Viral / chemistry
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Antigens, Viral / immunology*
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Bronchoalveolar Lavage Fluid / immunology
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Cell Wall / immunology
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Enzyme-Linked Immunosorbent Assay
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G(M1) Ganglioside / metabolism*
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Hemagglutinin Glycoproteins, Influenza Virus / administration & dosage
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Hemagglutinin Glycoproteins, Influenza Virus / immunology
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Immunoglobulin A / analysis
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Immunoglobulin G / analysis
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / immunology
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Mice
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Mice, Inbred BALB C
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Neuraminidase / administration & dosage
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Neuraminidase / immunology
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Neuraminidase / metabolism
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Peptide Library
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Peptides / administration & dosage
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Peptides / chemistry
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Peptides / immunology*
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Peptides / metabolism
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Protein Binding
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Streptococcus pneumoniae / immunology
Substances
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Adjuvants, Immunologic
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Antibodies, Bacterial
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Antibodies, Viral
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Antigens, Bacterial
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Antigens, Viral
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Hemagglutinin Glycoproteins, Influenza Virus
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Immunoglobulin A
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Immunoglobulin G
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Influenza Vaccines
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Peptide Library
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Peptides
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G(M1) Ganglioside
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Neuraminidase