Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars

FEBS Lett. 2005 Dec 19;579(30):6890-4. doi: 10.1016/j.febslet.2005.11.038. Epub 2005 Dec 1.

Abstract

Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 microM vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum / metabolism
  • Aluminum / pharmacology*
  • Ion Channels / classification
  • Ion Channels / drug effects
  • Ion Channels / metabolism*
  • Kinetics
  • Malates / metabolism
  • Membrane Potentials
  • Patch-Clamp Techniques
  • Plant Roots / cytology
  • Plant Roots / drug effects*
  • Plant Roots / metabolism
  • Time Factors
  • Triticum / genetics*
  • Triticum / metabolism*
  • Vacuoles / metabolism*

Substances

  • Ion Channels
  • Malates
  • Aluminum