Detection of Mycobacterium avium subsp. paratuberculosis in retail cheeses from Greece and the Czech Republic

Appl Environ Microbiol. 2005 Dec;71(12):8934-6. doi: 10.1128/AEM.71.12.8934-8936.2005.

Abstract

We investigated the presence of Mycobacterium avium subsp. paratuberculosis in retail cheeses from Greece and the Czech Republic. We found that 31.7% and 3.6% of our samples reacted positive by PCR and culture, respectively. Consumption of these cheeses is likely to result in human exposure to M. avium subsp. paratuberculosis, albeit at a low level for viable cells.

MeSH terms

  • Base Sequence
  • Cheese / microbiology*
  • Czech Republic
  • DNA Primers
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Electrophoresis, Agar Gel
  • Greece
  • Mycobacterium avium / genetics
  • Mycobacterium avium / isolation & purification*
  • Mycobacterium avium subsp. paratuberculosis / genetics
  • Mycobacterium avium subsp. paratuberculosis / isolation & purification*
  • Polymerase Chain Reaction

Substances

  • DNA Primers
  • DNA, Bacterial