Identification and characterization of RHEBL1, a novel member of Ras family, which activates transcriptional activities of NF-kappa B

Mol Biol Rep. 2005 Dec;32(4):205-14. doi: 10.1007/s11033-005-0984-x.

Abstract

The Ras family of small GTPases regulates a wide variety of cellular functions that include cell growth, differentiation, and transformation. In this study, we identified and characterized a novel member of Ras family named RHEBL1, belonging to the Rheb branch of small GTPase proteins. The cDNA sequence contains an open reading frame of 551 bp, encoding a putative protein of 183 amino acid residues. The expression pattern of RHEBL1 showed that it was ubiquitously expressed in 17 tissues. RHEBL1 gene encodes a 20.69 kDa protein, localized in cytoplasm when overexpressed in COS7 cells. Reporter gene assays showed that overexpression of RHEBL1 in HEK 293T cells strongly activated the transcriptional activities of NF-kappa B, while the mutant (D60K) only weakly activates NF-kappa B-mediated transcription. Our findings suggest that RHEBL1 is a positive regulator of NF-kappa B-mediated gene transcription.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • COS Cells
  • Chlorocebus aethiops
  • Cluster Analysis
  • DNA Primers
  • DNA, Complementary / genetics
  • Gene Components
  • Gene Expression*
  • Humans
  • Molecular Sequence Data
  • Mutagenesis
  • NF-kappa B / metabolism*
  • Phylogeny*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Transcriptional Activation / genetics*
  • ras Proteins / genetics*
  • ras Proteins / metabolism*

Substances

  • DNA Primers
  • DNA, Complementary
  • NF-kappa B
  • RHEBL1 protein, human
  • ras Proteins

Associated data

  • GENBANK/AY327412