Chemo-enzymatic synthesis of a trisaccharide-linked peptide aimed at improved drug-delivery

Ken D Johnstone; Manuela Dieckelmann; Michael P Jennings; Istvan Toth; Joanne T Blanchfield

doi:10.2174/1567201054367977

Chemo-enzymatic synthesis of a trisaccharide-linked peptide aimed at improved drug-delivery

Curr Drug Deliv. 2005 Jul;2(3):215-22. doi: 10.2174/1567201054367977.

Authors

Ken D Johnstone¹, Manuela Dieckelmann, Michael P Jennings, Istvan Toth, Joanne T Blanchfield

Affiliation

¹ School of Molecular and Microbial Sciences, University of Queensland, St. Lucia 4072, Qld, Australia.

PMID: 16305423
DOI: 10.2174/1567201054367977

Abstract

The glycosyltransferase enzyme LgtA, derived from the bacterial pathogen Neisseria meningitidis, was utilised in the chemo-enzymatic synthesis of a trisaccharide-linked endomorphin peptide drug candidate.

MeSH terms

Bacterial Proteins / chemistry*
Base Sequence
Carbohydrate Sequence
DNA Primers
DNA, Recombinant
Drug Delivery Systems*
Electrophoresis, Polyacrylamide Gel
Glycosylation
Molecular Sequence Data
N-Acetylglucosaminyltransferases / chemistry*
Peptides / administration & dosage
Peptides / chemistry*
Spectrometry, Mass, Electrospray Ionization
Trisaccharides / administration & dosage
Trisaccharides / chemistry*

Substances

Bacterial Proteins
DNA Primers
DNA, Recombinant
Peptides
Trisaccharides
LgtA protein, bacteria
N-Acetylglucosaminyltransferases