Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone

Biochem Biophys Res Commun. 2006 Jan 6;339(1):362-6. doi: 10.1016/j.bbrc.2005.11.035. Epub 2005 Nov 14.

Abstract

The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs--idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ1 were found to be more efficient in the scavenging activity (IC50: 0.052 and 0.075 microM, respectively) than CoQ3 (IC50: 45.8 microM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / metabolism*
  • Adipose Tissue, Brown / ultrastructure
  • Animals
  • Antioxidants / pharmacology*
  • Benzoquinones / pharmacology*
  • Cricetinae
  • Ferricyanides / pharmacology
  • Free Radical Scavengers / pharmacology*
  • Glycerophosphates / metabolism*
  • Hydrogen Peroxide / metabolism*
  • In Vitro Techniques
  • Male
  • Mesocricetus
  • Mitochondria / drug effects*
  • Mitochondria / metabolism
  • Oxygen Consumption / drug effects
  • Reactive Oxygen Species / metabolism
  • Succinates / metabolism
  • Ubiquinone / metabolism

Substances

  • Antioxidants
  • Benzoquinones
  • Ferricyanides
  • Free Radical Scavengers
  • Glycerophosphates
  • Reactive Oxygen Species
  • Succinates
  • ubiquinone Q3
  • Ubiquinone
  • hexacyanoferrate III
  • Hydrogen Peroxide
  • idebenone
  • Ubiquinone Q1