Attenuation of Salmonella enterica Serovar Typhimurium by altering biological functions of murein lipoprotein and lipopolysaccharide

A A Fadl; J Sha; G R Klimpel; J P Olano; C L Galindo; A K Chopra

doi:10.1128/IAI.73.12.8433-8436.2005

Attenuation of Salmonella enterica Serovar Typhimurium by altering biological functions of murein lipoprotein and lipopolysaccharide

Infect Immun. 2005 Dec;73(12):8433-6. doi: 10.1128/IAI.73.12.8433-8436.2005.

Authors

A A Fadl¹, J Sha, G R Klimpel, J P Olano, C L Galindo, A K Chopra

Affiliation

¹ Department of Microbiology and Immunology, 301 University Blvd., University of Texas Medical Branch, Galveston, TX 77555-1070, USA.

Abstract

We constructed Salmonella enterica serovar Typhimurium double-knockout mutants in which either the lipoprotein A (lppA) or the lipoprotein B (lppB) gene was deleted from an msbB-negative background strain by marker exchange mutagenesis. These mutants were highly attenuated when tested with in vitro and in vivo models of Salmonella pathogenesis.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acyltransferases / genetics
Animals
Bacterial Proteins / genetics*
Bacterial Proteins / immunology
Gene Deletion
Immune Sera / immunology
Lipopolysaccharides / immunology
Lipoprotein(a) / genetics
Lipoprotein(a) / immunology
Lipoproteins / genetics*
Lipoproteins / immunology
Mice
Mutation
Salmonella Infections / immunology
Salmonella Infections / microbiology*
Salmonella typhimurium / genetics
Salmonella typhimurium / immunology
Salmonella typhimurium / pathogenicity*

Substances

Bacterial Proteins
Immune Sera
Lipopolysaccharides
Lipoprotein(a)
Lipoproteins
Acyltransferases