Efficient spin-spin scalar coupling mediated C-13 homonuclear polarization transfer in biological solids without proton decoupling

Yun Mou; John Chin Hao Chao; Jerry C C Chan

doi:10.1016/j.ssnmr.2005.10.004

Efficient spin-spin scalar coupling mediated C-13 homonuclear polarization transfer in biological solids without proton decoupling

Solid State Nucl Magn Reson. 2006 Jun;29(4):278-82. doi: 10.1016/j.ssnmr.2005.10.004. Epub 2005 Nov 10.

Authors

Yun Mou¹, John Chin Hao Chao, Jerry C C Chan

Affiliation

¹ Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei, Taiwan.

PMID: 16289520
DOI: 10.1016/j.ssnmr.2005.10.004

Abstract

We demonstrate that an efficient C' <--> C alpha polarization transfer based on J-coupling can be realized under fast magic-angle spinning (MAS) condition without 1H decoupling. Experimental results are presented for model crystalline compounds as well as a non-crystalline 17-residue polypeptide MB(i + 4)EK. Measurements on MB(i + 4)EK demonstrate that 53% of the initial C' polarization was transferred to the cross peaks at 7.05 T under 25 kHz MAS spinning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alanine / analysis*
Biopolymers / analysis*
Carbon Isotopes
Nuclear Magnetic Resonance, Biomolecular / methods*
Peptides / analysis*
Protons
Spin Labels
Valine / analysis*

Substances

Biopolymers
Carbon Isotopes
Peptides
Protons
Spin Labels
Valine
Alanine