An integrated optical leaky waveguide sensor with electrically induced concentration system for the detection of bacteria

Lab Chip. 2005 Dec;5(12):1360-5. doi: 10.1039/b504938e. Epub 2005 Oct 6.

Abstract

An integrated, sensitive and rapid system was developed for the detection of bacteria. The system combined an optical metal-clad leaky waveguide (MCLW) sensor with an electric field. The electric field was used to concentrate Bacillus subtilis var. niger(BG) bacteria spores onto the immobilized anti-BG antibody on the MCLW sensor surface. This sensor combination has been characterised by detecting the scattering from bacterial spores, which are concentrated at the sensor surface, when they are illuminated at the coupling angle; and by detection of fluorescence from labelled antibodies added after the spores had been captured on the surface. The light scattering and fluorescence detection methods gave a detection limit of BG bacterial spores of 1 x 10(3) spores ml(-1) when the electric field was applied for 3 minutes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / isolation & purification*
  • Colony Count, Microbial / instrumentation*
  • Colony Count, Microbial / methods
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Flow Cytometry / instrumentation*
  • Flow Cytometry / methods
  • Immunoassay / instrumentation
  • Immunoassay / methods
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Optics and Photonics / instrumentation*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / instrumentation*
  • Spectrometry, Fluorescence / methods
  • Spores / isolation & purification
  • Systems Integration
  • Transducers*