G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs

Hidetoshi Tahara; Mayuko Kusunoki; Yusuke Yamanaka; Shusaku Matsumura; Toshinori Ide

doi:10.1038/nmeth797

G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs

Nat Methods. 2005 Nov;2(11):829-31. doi: 10.1038/nmeth797.

Authors

Hidetoshi Tahara¹, Mayuko Kusunoki, Yusuke Yamanaka, Shusaku Matsumura, Toshinori Ide

Affiliation

¹ Department of Cellular and Molecular Biology, Division of Integrated Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8551, Japan. toshi@hiroshima-u.ac.jp

PMID: 16278652
DOI: 10.1038/nmeth797

Abstract

Accurate measurement of telomeric 3'-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cell Line, Tumor
DNA, Single-Stranded / analysis*
DNA, Single-Stranded / genetics
Humans
Nucleic Acid Hybridization / methods*
Repetitive Sequences, Nucleic Acid / genetics
Sensitivity and Specificity
Telomere / chemistry
Telomere / genetics*

Substances

DNA, Single-Stranded