[Detection and identification of phytoplasma strain associated with periwinkle yellows]

Hong Cai; Baohua Kong; Hairu Chen

[Detection and identification of phytoplasma strain associated with periwinkle yellows]

Wei Sheng Wu Xue Bao. 2003 Feb;43(1):116-9.

[Article in Chinese]

Authors

Hong Cai¹, Baohua Kong, Hairu Chen

Affiliation

¹ Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China.

PMID: 16276881

Abstract

A 1.2kb and 1.3kb DNA fragments were amplified in DNA samples extracted from periwinkle exhibiting yellows naturally. The amplified fragments were cloned, sequenced and compared with other phytoplasma strains. The results showed that the 16S rDNA and ribosomal protein gene of periwinkle yellows phytoplasma consist of 1239bp and 1244bp, respectively. They all shared high homology (higher than 97.5%) with phytoplasma strains of 16S rI group. Thus, the result made it clear that the phytoplasma strain of periwinkle yellows (PY) is one of the members of phytoplasma 16SrI group.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Catharanthus / microbiology*
Phytoplasma / classification
Phytoplasma / genetics
Phytoplasma / isolation & purification*
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics

Substances

RNA, Ribosomal, 16S