Abstract
We have identified two types of homologous DNA pairing activity in mouse cell extracts by a strand-transfer assay. Both activities are separated from each other by anion-exchange chromatography; neither of them needs ATP. One requires magnesium ion and is stimulated by Escherichia coli single-stranded DNA binding protein, whereas the other does not require the ion and shows a higher affinity for a left-handed Z-DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Animals
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Chromatography, Affinity
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Chromatography, Ion Exchange
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Clone Cells
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DNA, Bacterial / genetics
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DNA, Bacterial / metabolism*
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DNA, Circular / genetics
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DNA, Circular / metabolism*
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DNA, Single-Stranded / genetics
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DNA, Single-Stranded / metabolism*
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DNA-Binding Proteins / metabolism*
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Escherichia coli / metabolism
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Magnesium / metabolism
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Mammary Neoplasms, Experimental
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Mice
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Substrate Specificity
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Tumor Cells, Cultured
Substances
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DNA, Bacterial
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DNA, Circular
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DNA, Single-Stranded
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DNA-Binding Proteins
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Adenosine Triphosphate
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Magnesium