Identification of two types of homologous DNA pairing activity in mouse cells

Biochem Biophys Res Commun. 1992 Jun 30;185(3):987-92. doi: 10.1016/0006-291x(92)91724-5.

Abstract

We have identified two types of homologous DNA pairing activity in mouse cell extracts by a strand-transfer assay. Both activities are separated from each other by anion-exchange chromatography; neither of them needs ATP. One requires magnesium ion and is stimulated by Escherichia coli single-stranded DNA binding protein, whereas the other does not require the ion and shows a higher affinity for a left-handed Z-DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Chromatography, Affinity
  • Chromatography, Ion Exchange
  • Clone Cells
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism*
  • DNA, Circular / genetics
  • DNA, Circular / metabolism*
  • DNA, Single-Stranded / genetics
  • DNA, Single-Stranded / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / metabolism
  • Magnesium / metabolism
  • Mammary Neoplasms, Experimental
  • Mice
  • Substrate Specificity
  • Tumor Cells, Cultured

Substances

  • DNA, Bacterial
  • DNA, Circular
  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • Adenosine Triphosphate
  • Magnesium