Heterogeneity of putative surface layer proteins in Lactobacillus helveticus

Appl Environ Microbiol. 2005 Nov;71(11):7582-8. doi: 10.1128/AEM.71.11.7582-7588.2005.

Abstract

The S-layer-encoding genes of 21 Lactobacillus helveticus strains were characterized. Phylogenetic analysis based on the identified S-layer genes revealed two main clusters, one which includes a sequence similar to that of the slpH1 gene of L. helveticus CNRZ 892 and a second cluster which includes genes similar to that of prtY. These results were further confirmed by Southern blot hybridization. This study demonstrates S-layer gene variability in the species L. helveticus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Blotting, Southern
  • Genetic Variation*
  • Lactobacillus helveticus / classification*
  • Lactobacillus helveticus / genetics*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Multigene Family
  • Phylogeny
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins
  • Membrane Glycoproteins
  • S-layer proteins