Modified binary hammerhead ribozymes with high catalytic activity

Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):1105-9. doi: 10.1081/ncn-200060181.

Abstract

A series of binary hammerhead ribozymes was designed and assessed in terms of cleavage activity and nuclease resistance. Enhanced nuclease resistance of binary ribozymes was achieved by incorporation of Z-modified nucleotides at the selective positions along with addition of 3'-3-linked thymidine cap. These modified binary ribozymes efficiently cleave 190-nucleotides long MDR1 mRNA fragment and display catalytic activity much higher then respective full-length analogs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • Catalysis
  • Genes, MDR / genetics
  • Genetic Techniques
  • Kinetics
  • Molecular Biology / methods*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Protein Engineering / methods
  • RNA, Catalytic / chemistry*
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • Substrate Specificity
  • Temperature
  • Time Factors

Substances

  • RNA, Catalytic
  • RNA, Messenger
  • hammerhead ribozyme