Microwaves synthesis of solid supports for the synthesis of 3'-aminoalkyl oligodeoxynucleotides

Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):623-7. doi: 10.1081/ncn-200059394.

Abstract

Phthalimido-alkyl alcohol solid supports were rapidly prepared from solid supported phthalic anhydride and amino alcohol condensation induced by microwaves. These supports were used to synthesize 13-aminoalkyl oligodeoxynucleotides allowing a two step deprotection necessary to avoid aminolink alkylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • DNA / chemistry
  • Microwaves*
  • Models, Chemical
  • Molecular Biology / methods*
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Oligonucleotides / radiation effects
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Time Factors
  • Ultraviolet Rays

Substances

  • Oligonucleotides
  • DNA