Abstract
Viscum album agglutinin-I (VAA-I) is a potent inducer of cell apoptosis and possesses anti-tumoral activity. Using PLB-985 and chronic granulomatous disease (X-CGD) cells, which lack expression of gp91(phox), VAA-I was found to induce apoptosis in both cell lines as assessed by cytology, DNA laddering and degradation of the cytoskeletal protein gelsolin. Both cell lines expressed caspase-3 and -8 and VAA-I activated these caspases. We demonstrated that lamin B(1) is a novel target to VAA-I and its degradation was reversed by a pan-caspase inhibitor and by a caspase-6, but not a caspase-8, inhibitor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects*
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Caspase 3
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Caspase 8
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Caspases / drug effects
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Caspases / metabolism
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Cell Line, Tumor
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Cytoskeletal Proteins / drug effects*
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Cytoskeletal Proteins / metabolism
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Gelsolin / antagonists & inhibitors
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Humans
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Lamin Type B / antagonists & inhibitors
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Leukemia / drug therapy
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Leukemia / pathology*
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Plant Lectins / pharmacology*
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Plant Preparations / pharmacology*
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Plant Proteins / pharmacology*
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Ribosome Inactivating Proteins
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Ribosome Inactivating Proteins, Type 2
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Toxins, Biological / pharmacology*
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Viscum album / chemistry
Substances
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Cytoskeletal Proteins
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Gelsolin
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Lamin Type B
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Plant Lectins
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Plant Preparations
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Plant Proteins
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Ribosome Inactivating Proteins, Type 2
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Toxins, Biological
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VAA-I protein, Viscum album
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Ribosome Inactivating Proteins
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CASP3 protein, human
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CASP8 protein, human
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Caspase 3
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Caspase 8
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Caspases