Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD

Shigetarou Mori; Masayuki Yamasaki; Yukie Maruyama; Keiko Momma; Shigeyuki Kawai; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata

doi:10.1016/S1389-1723(04)00302-0

Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD

J Biosci Bioeng. 2004;98(5):391-3. doi: 10.1016/S1389-1723(04)00302-0.

Authors

Shigetarou Mori¹, Masayuki Yamasaki, Yukie Maruyama, Keiko Momma, Shigeyuki Kawai, Wataru Hashimoto, Bunzo Mikami, Kousaku Murata

Affiliation

¹ Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.

PMID: 16233726
DOI: 10.1016/S1389-1723(04)00302-0

Abstract

NAD kinase from Mycobacterium tuberculosis (Ppnk) uses ATP or inorganic polyphosphate [poly(P)]. Ppnk overexpressed in Escherichia coli was purified and crystallized in the presence of NAD. Preliminary X-ray analysis of the resultant crystal indicate that the crystal belongs to hexagonal space group P6(2)22 and is holo-Ppnk complexed with NAD.