Overexpression and purification of microbial pro-transglutaminase from Streptomyces cinnamoneum and in vitro processing by Streptomyces albogriseolus proteases

Seiichi Taguchi; Kumiko Arakawa; Keiichi Yokoyama; Shino Takehana; Hiroshi Takagi; Haruo Momose

doi:10.1016/s1389-1723(02)80228-6

Overexpression and purification of microbial pro-transglutaminase from Streptomyces cinnamoneum and in vitro processing by Streptomyces albogriseolus proteases

J Biosci Bioeng. 2002;94(5):478-81. doi: 10.1016/s1389-1723(02)80228-6.

Authors

Seiichi Taguchi¹, Kumiko Arakawa, Keiichi Yokoyama, Shino Takehana, Hiroshi Takagi, Haruo Momose

Affiliation

¹ School of Agriculture, Meiji University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki, Kamagawa 214-8571, Japan. staguchi@isc.meiji.ac.jp

PMID: 16233337
DOI: 10.1016/s1389-1723(02)80228-6

Abstract

Processing and activation of the precursor of an extracellular Streptomyces transglutaminase were achieved by using three Streptomyces proteases (SAM-P20, SAM-P26 and SAM-P45), all of which are widely distributed in Streptomyces. The use of these proteases would allow us to develop a production process for the active form of this enzyme in recombinant bacteria.