Molecular cloning and characterization of two cDNAs encoding asparagine synthetase from Astragalus sinicus nodules

K Kasai; M Fujie; Y Nakanishi; Y Murooka; S Usami; T Yamada

doi:10.1016/s1389-1723(00)80057-2

Molecular cloning and characterization of two cDNAs encoding asparagine synthetase from Astragalus sinicus nodules

J Biosci Bioeng. 2000;89(6):559-63. doi: 10.1016/s1389-1723(00)80057-2.

Authors

K Kasai¹, M Fujie, Y Nakanishi, Y Murooka, S Usami, T Yamada

Affiliation

¹ Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8527, Japan.

PMID: 16232798
DOI: 10.1016/s1389-1723(00)80057-2

Abstract

Two cDNAs that encode asparagine synthetase were cloned from root nodules of Astragalus sinicus cv. Japan (Renge-sou). The expression of the transcripts was nodule-enhanced. The expression of both genes was reduced in nodules when (NH4)2SO4 was added to the culture medium. This is the first report of the inhibition of asparagine synthetase gene expression by ammonium in root nodules.