Objective: To study the effects of sterigmatocystin (ST) on HLA-I of human peripheral blood mononuclear cells (HPBMc) in vitro.
Methods: The expression of HLA- I of HPBMc by ST pretreatment at six different dosages (0, 0.125, 0.25, 0.5, 1, 2 mg/L) were detected with Flow cytometry (FCM) and Western blot respectively.
Results: FCM quantitative analysis revealed that the expressions of HLA-I of HPBMc in vitro as represented by fluorescence intensity after ST treatment for 24 hrs at all the five experimental concentrations were all lower than that of the control group. The inhibiting effects at relatively higher concentration groups (0.5, 1 and 2 mg/L) were of significance (P < 0.05). A dose-effect relationship between ST concentration and the inhibiting effect on HLA-I expression was found at the concentration range from 0.125mg/L to 2 mg/L (r = 0.841, P < 0.01). Western Blotting results further confirmed the above results.
Conclusion: Within the dosage range from 0.125 mg/L to 2 mg/L, ST could inhibit the expression of HLA- I of HPBMc in vitro negative dose-dependently.