Nematodes represent a very abundant group of soil organisms and non-parasitic species are important for soil quality and in the soil food web. In recent years, it has been shown that nematodes are appropriate bioindicators of soil condition and they are also suitable organisms for laboratory toxicity testing. The aims of this paper are to overview and critically assess methods and approaches for researching soil nematode ecotoxicology. In natural ecosystems, nematode abundance and community structure analyses were proved to be sensitive indicators of stress caused by soil pollutants and ecological disturbance. Community structure analyses may be approached from a functional or ecological point of view; species are divided into groups according to their feeding habits or alternatively the maturity index is calculated according to their ecological strategy. Many environmental factors have the potential to affect nematode community, which consequently results in high space and time variability. This variance is major handicap in field ecotoxicological studies because pollutant-nematode relationships are obscured. For prospective risk assessment of chemicals, several toxicity tests with nematodes were developed and are increasingly used. Sensitivity of these tests is comparable to tests with other soil species (e.g. enchytraeids, earthworms and springtails) while tests are less demanding to space and time. Most studies have focused on metal toxicity but organic compounds are almost overlooked. Endpoints used in tests were often mortality, reproduction or movement, but more sublethal endpoints such as feeding or biomarkers have been used recently too. Although there is an increasing amount of knowledge in soil nematode ecotoxicology, there is still a lot of various issues in this topic to research.