Characterization of vacuolar membrane proteins changed in rice root treated with gibberellin

J Proteome Res. 2005 Sep-Oct;4(5):1775-80. doi: 10.1021/pr050079c.

Abstract

Rice vacuolar membrane proteins changed by gibberellin (GA) were analyzed using a proteome approach. Vacuolar membrane fractions were isolated using a discontinuous sucrose/sorbitol system and 10 proteins increased in vacuolar membrane of the root, treated with GA(3) as compared with control. Fructose-1,6-bisphosphate aldolase C-1 and vacuolar H(+)-ATPase (V-ATPase) increased in root vacuolar membrane by GA(3) interacted in rice roots. It suggests that aldolase C-1 regulates the V-ATPase mediated control of cell elongation that determines root growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / chemistry
  • Amino Acid Sequence
  • Blotting, Western
  • Cell Membrane / metabolism*
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Inhibitors / pharmacology
  • Fructosediphosphates / chemistry
  • Gibberellins / chemistry*
  • Immunoprecipitation
  • Isoelectric Focusing
  • Models, Biological
  • Molecular Sequence Data
  • Oryza / genetics
  • Oryza / metabolism*
  • Peptide Mapping
  • Peptides / chemistry
  • Plant Physiological Phenomena
  • Plant Proteins / chemistry
  • Plant Roots / chemistry*
  • Plants, Genetically Modified / genetics
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Silver Staining
  • Sorbitol / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sucrose / chemistry
  • Temperature
  • Vacuolar Proton-Translocating ATPases / chemistry
  • Vacuoles / chemistry*

Substances

  • Enzyme Inhibitors
  • Fructosediphosphates
  • Gibberellins
  • Peptides
  • Plant Proteins
  • Sorbitol
  • Sucrose
  • Adenosine Triphosphatases
  • Vacuolar Proton-Translocating ATPases
  • fructose-1,6-diphosphate