Abstract
Rice vacuolar membrane proteins changed by gibberellin (GA) were analyzed using a proteome approach. Vacuolar membrane fractions were isolated using a discontinuous sucrose/sorbitol system and 10 proteins increased in vacuolar membrane of the root, treated with GA(3) as compared with control. Fructose-1,6-bisphosphate aldolase C-1 and vacuolar H(+)-ATPase (V-ATPase) increased in root vacuolar membrane by GA(3) interacted in rice roots. It suggests that aldolase C-1 regulates the V-ATPase mediated control of cell elongation that determines root growth.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / chemistry
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Amino Acid Sequence
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Blotting, Western
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Cell Membrane / metabolism*
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Electrophoresis, Gel, Two-Dimensional
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Electrophoresis, Polyacrylamide Gel
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Enzyme Inhibitors / pharmacology
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Fructosediphosphates / chemistry
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Gibberellins / chemistry*
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Immunoprecipitation
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Isoelectric Focusing
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Models, Biological
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Molecular Sequence Data
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Oryza / genetics
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Oryza / metabolism*
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Peptide Mapping
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Peptides / chemistry
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Plant Physiological Phenomena
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Plant Proteins / chemistry
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Plant Roots / chemistry*
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Plants, Genetically Modified / genetics
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Sequence Homology, Amino Acid
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Signal Transduction
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Silver Staining
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Sorbitol / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Sucrose / chemistry
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Temperature
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Vacuolar Proton-Translocating ATPases / chemistry
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Vacuoles / chemistry*
Substances
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Enzyme Inhibitors
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Fructosediphosphates
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Gibberellins
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Peptides
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Plant Proteins
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Sorbitol
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Sucrose
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Adenosine Triphosphatases
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Vacuolar Proton-Translocating ATPases
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fructose-1,6-diphosphate