Long chain fatty acyl-CoA synthetase 5 expression is induced by insulin and glucose: involvement of sterol regulatory element-binding protein-1c

Biochimie. 2005 Dec;87(12):1149-55. doi: 10.1016/j.biochi.2005.04.015. Epub 2005 Sep 20.

Abstract

In a screen for sterol regulatory element-binding protein (SREBP)-1c target genes in the liver, we identified long chain fatty acyl-CoA synthetase 5 (ACS-5). Hepatic ACS-5 mRNA is poorly expressed during fasting and diabetes and strongly induced by carbohydrate refeeding and insulin treatment. In cultured hepatocytes, insulin and a high glucose concentration induce ACS-5 mRNA. Adenoviral overexpression of a nuclear form of SREBP-1c in liver of diabetic mice or in cultured hepatocytes mimics the effect of insulin to induce ACS-5. By contrast, a dominant negative form of SREBP-1c abolishes the effect of insulin on ACS-5 expression. The dietary and SREBP-1c-mediated insulin regulation of ACS-5 expression indicate that ACS-5 is involved in the anabolic fate of fatty acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coenzyme A Ligases / biosynthesis*
  • Coenzyme A Ligases / drug effects
  • Coenzyme A Ligases / genetics*
  • Coenzyme A Ligases / metabolism*
  • Diabetes Mellitus, Experimental / physiopathology*
  • Eating
  • Enzyme Induction
  • Fasting
  • Fatty Acids / metabolism
  • Female
  • Glucose / pharmacology*
  • Insulin / pharmacology*
  • Liver / enzymology
  • Mitochondrial Proteins
  • Models, Animal
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar
  • Sterol Regulatory Element Binding Protein 1 / physiology*

Substances

  • Fatty Acids
  • Insulin
  • Mitochondrial Proteins
  • RNA, Messenger
  • Sterol Regulatory Element Binding Protein 1
  • Coenzyme A Ligases
  • Acsl5 protein, rat
  • Glucose