High-level production of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 using statistical experimental designs

Biosci Biotechnol Biochem. 2005 Sep;69(9):1743-8. doi: 10.1271/bbb.69.1743.

Abstract

Medium composition was optimized for high-level production of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 using statistical experimental designs. Glucose and yeast extract were the most important factors affecting astaxanthin production. Glucose 3.89%, yeast extract 0.29%, KH2PO4 0.25%, MgSO4 0.05%, MnSO4 0.02%, and CaCl2 0.01% were optimum for high-level production of astaxanthin. Under optimized conditions, the maximum concentration of astaxanthin obtained after 7 d of cultivation was 36.06 mg/l. The concentration of astaxanthin predicted by a polynomial model was 36.16 mg/l.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / genetics*
  • Culture Media / chemistry
  • Data Interpretation, Statistical
  • Glucose / pharmacology
  • Models, Statistical*
  • Mutation
  • Research Design
  • Xanthophylls
  • Yeasts / chemistry
  • beta Carotene / analogs & derivatives*
  • beta Carotene / analysis
  • beta Carotene / biosynthesis

Substances

  • Culture Media
  • Xanthophylls
  • beta Carotene
  • astaxanthine
  • Glucose