Molecular tests can allow confirmation of invasive meningococcal disease when isolates yield atypical maltose, glucose or gamma-glutamyl peptidase test results

Angelo Zaia; Julia M Griffith; Tiffany R Hogan; John W Tapsall; Peter Bainbridge; Rachel Neill; David Tribe

doi:10.1080/00313020500254586

Molecular tests can allow confirmation of invasive meningococcal disease when isolates yield atypical maltose, glucose or gamma-glutamyl peptidase test results

Pathology. 2005 Oct;37(5):378-9. doi: 10.1080/00313020500254586.

Authors

Angelo Zaia¹, Julia M Griffith, Tiffany R Hogan, John W Tapsall, Peter Bainbridge, Rachel Neill, David Tribe

Affiliation

¹ Microbiological Diagnostic Unit Public Health Laboratory, University of Melbourne, Parkville, Victoria, Australia.

PMID: 16194849
DOI: 10.1080/00313020500254586

Abstract

Analysis of atypical meningococci from invasive disease that either (i) did not produce acid from maltose and glucose or (ii) were gamma-glutamyl peptidase test negative for porA and porB DNA variable region (VR) type, multilocus sequence type, and for presence of capsule transport gene ctrA, conclusively demonstrated that these are Neisseria meningitidis.

MeSH terms

DNA, Bacterial / analysis
Glucose / metabolism*
Humans
Immunoglobulin Variable Region
Maltose / metabolism*
Meningitis, Meningococcal / diagnosis*
Meningitis, Meningococcal / microbiology
Neisseria meningitidis / genetics
Neisseria meningitidis / isolation & purification*
Neisseria meningitidis / metabolism
Polymerase Chain Reaction / methods*
Porins / classification
Porins / genetics
Serotyping*
gamma-Glutamyltransferase / metabolism*

Substances

DNA, Bacterial
Immunoglobulin Variable Region
Porins
porin protein, Neisseria
Maltose
gamma-Glutamyltransferase
Glucose