Optical leaky waveguide sensor for detection of bacteria with ultrasound attractor force

Mohammed Zourob; Jeremy J Hawkes; W Terence Coakley; Bernard J Treves Brown; Peter R Fielden; Martin B McDonnell; Nicholas J Goddard

doi:10.1021/ac050605j

Optical leaky waveguide sensor for detection of bacteria with ultrasound attractor force

Anal Chem. 2005 Oct 1;77(19):6163-8. doi: 10.1021/ac050605j.

Authors

Mohammed Zourob¹, Jeremy J Hawkes, W Terence Coakley, Bernard J Treves Brown, Peter R Fielden, Martin B McDonnell, Nicholas J Goddard

Affiliation

¹ School of Chemical Engineering and Analytical Science, University of Manchester, P.O. Box 88, Sackville Street, Manchester M60 1QD, UK. m.zourob@manchester.ac.uk

PMID: 16194074
DOI: 10.1021/ac050605j

Abstract

An integrated, sensitive, and rapid system was developed for the detection of bacteria. The system combined an optical metal-clad leaky waveguide (MCLW) sensor with ultrasound standing waves (USW). The performance of a MCLW sensor for the detection of bacteria has been increased (>100 fold) by using USWs to drive bacteria onto the sensor surface. By forming the USW nodes at or within the surface of the MCLW, the diffusion-limited capture rate has been replaced by fast movement. Immobilized anti-BG antibody on the MCLW sensor surface was used to capture Bacillus subtilis var. niger (BG) bacterial spores driven to the surface. This combination of sensor and attractor force combination has been tested by detecting the evanescent scattering from bacterial spores at the sensor surface. Application of ultrasound for 3 min gave a detection limit for BG bacterial spores of 1 x 10(3) spores/mL.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / ultrastructure*
Biosensing Techniques / instrumentation*
Biosensing Techniques / methods*
Optics and Photonics*
Spores, Bacterial