Purpose: The aim of this study is to evaluate possible morphometric alterations in bone tissue and the calcium serum concentration in ovariectomized mice subjected to physical training.
Methods: Fifty female mice, aged 90 days, divided into 5 groups (n=10): control (C), pseudo-operated sedentary (POS), pseudo-operated trained (POT), ovariectomized sedentary (OVS) and ovariectomized trained (OVT). Groups OVS and OVT were subjected to an ovariectomy, and groups POS and POT were subjected to a pseudo-surgery. Thirty days after the surgery, groups POT and OVT were subjected to physical training during 05 weeks in ergometer at a speed of 20 m/min. The other animals stayed sedentary at the same period. Afterwards the animals were sacrificed and had blood collected to realize calcium serum dosage and the right femurs were collected to realize a histomorphometric study.
Results: The calcium serum concentration in group OVT was lower than the other groups (p<0.05). The mass of femurs in groups POT and OVT was higher than the group Control (p<0.05). The average density of the osteocytes was higher in group OVS (p<0.05). The average value of the area of the osteocytes showed difference only between groups POS and OVS (p<0.05). There were no differences in the bone length, nor in the osteocytes perimeter.
Conclusion: The physical training in part prevented the alterations in the bone tissue due to the ovariectomy and enabled an increase of the bone formation.