Abstract
Objective:
To investigate the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in the tissues of normal adrenal medulla and pheochromocytoma.
Methods:
Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA.
Results:
Expressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48+/-0.09 and 0.75+/-0.24, RAMP2/ GAPDH 0.79+/-0.12 and 1.29+/-0.30, CRLR/GAPDH 0.40+/-0.08 and 0.87+/-0.22 in normal adrenal medulla and pheochromocytomas, respectively (P < 0.05).
Conclusion:
ADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenal Gland Neoplasms / metabolism*
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Adrenal Medulla / metabolism*
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Adrenomedullin
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Adult
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Calcitonin Gene-Related Peptide / biosynthesis
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Calcitonin Gene-Related Peptide / genetics
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Female
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Male
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics
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Middle Aged
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Peptides / genetics
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Peptides / metabolism*
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Pheochromocytoma / metabolism*
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Receptor Activity-Modifying Protein 2
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Receptor Activity-Modifying Proteins
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Receptors, Adrenomedullin
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Receptors, Calcitonin / biosynthesis
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Receptors, Calcitonin / genetics
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Receptors, Peptide / metabolism*
Substances
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Peptides
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RAMP2 protein, human
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RNA, Messenger
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Receptor Activity-Modifying Protein 2
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Receptor Activity-Modifying Proteins
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Receptors, Adrenomedullin
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Receptors, Calcitonin
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Receptors, Peptide
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Adrenomedullin
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Calcitonin Gene-Related Peptide