[Expression of adrenomedullin and its receptor mRNA in the tissues of normal adrenal medulla and pheochromocytoma]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Aug;27(4):452-6.
[Article in Chinese]

Abstract

Objective: To investigate the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in the tissues of normal adrenal medulla and pheochromocytoma.

Methods: Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA.

Results: Expressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48+/-0.09 and 0.75+/-0.24, RAMP2/ GAPDH 0.79+/-0.12 and 1.29+/-0.30, CRLR/GAPDH 0.40+/-0.08 and 0.87+/-0.22 in normal adrenal medulla and pheochromocytomas, respectively (P < 0.05).

Conclusion: ADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Gland Neoplasms / metabolism*
  • Adrenal Medulla / metabolism*
  • Adrenomedullin
  • Adult
  • Calcitonin Gene-Related Peptide / biosynthesis
  • Calcitonin Gene-Related Peptide / genetics
  • Female
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics
  • Male
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics
  • Middle Aged
  • Peptides / genetics
  • Peptides / metabolism*
  • Pheochromocytoma / metabolism*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptor Activity-Modifying Protein 2
  • Receptor Activity-Modifying Proteins
  • Receptors, Adrenomedullin
  • Receptors, Calcitonin / biosynthesis
  • Receptors, Calcitonin / genetics
  • Receptors, Peptide / metabolism*

Substances

  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Peptides
  • RAMP2 protein, human
  • RNA, Messenger
  • Receptor Activity-Modifying Protein 2
  • Receptor Activity-Modifying Proteins
  • Receptors, Adrenomedullin
  • Receptors, Calcitonin
  • Receptors, Peptide
  • Adrenomedullin
  • Calcitonin Gene-Related Peptide